This second editions explores new and updated approaches for enrichment, detection, isolation, and molecular profiling of circulating tumor cells (CTCs). Chapters detail comprehensive protocols for the capture of CTCs via filtration, density gradient centrifugation, microfluidic, immunomagnetic separation of CTCs, detection of CTCs by immunocytochemistry, fluorescence in situ hybridization, flow cytometry, assays designed for genomic characterization, and functional analyses of CTCs. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights **tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Circulating Tumor Cells: Methods and Protocols, Second Edition aims to be comprehensive guide for **researchers in the field.

Inhalt

Detection and Separation of Circulating Tumor Cells Using Magnetic Fluorescent Nanoparticles.- Optimized Workflow for Sensitive Detection of Circulating Tumor Cells Using qPCR and Pre-Amplification to Minimize Cross-Contamination.- Highly efficient capture of sarcoma cells in microfluidics devices.- Detection of Circulating tumor cells with an Epithelial-to-Mesenchymal Transition Phenotype.- Phenotypic characterization and sorting of circulating tumor cells (CTCs) using a digital image-based sorter.- A New Filter for Isolation of Circulating Tumor Cells by Only Blood Filtration.- Androgen Receptor Variant 7 (AR-V7) transcript expression in circulating tumor cells.- Androgen Receptor Variant 7 (AR-V7) protein detection in circulating tumor cells by immunocytostaining.- Androgen receptor splice variant-7 expression analysis in circulating tumor cells: methodology and application of AdnaTest in prostate cancer research.- Nanomechanical Phenotyping of Circulating Tumor Cells with atomic force microscopy.- Model Circulating Tumor Cells Induced by Mechanical Stress.- Characterization of circulating tumor cells and other rare cells in blood using the RareCyte platform.- Liquid biopsies and molecular imaging for precise cancer management.- Small cell lung cancer: screening of kinase inhibitors.- Cell-interaction analysis and functional studies using circulating tumor cell models.-Negative Enrichment and Isolation of Circulating Tumor Cells from Cryopreserved Blood Fractions for Whole Genome Amplification.- Isolation and sequencing of single circulating tumor cells.- Micro RNA Sequencing in circulating tumor cells.- Single-cell RNA sequencing of pancreatic circulating tumor cells.- Utilizing magnetic microraft technology for single-cell isolation of CTCs from whole blood for scRNA-seq transcriptomic characterization .- Advancements in circulating tumor cell expansion ex vivo: a matured technology?.- Culture and expansion of circulating tumor cells from breast malignant effusions as organoids.- Bioinformatics of shallow whole genome sequencing data of circulating tumor cells to inform cancer diagnosis and treatment.-mAutomated analysis of liquid biopsy using deep learning: detecting circulating tumor cells and cancer-associated fibroblasts.- Artificial Intelligence for CELLSEARCH image analysis.- RNA-Seq and XAI can be used as tools to aid pathologists in the process of cancer diagnosis.- New strategies to uncover genes involved in the development of prostate cancer in families with multiple cases.